Immune Monitoring Products

Controls

ImmunoSite Technologies, LLC (IST) specializes in the development of unique control cell products.

CD4 Sure Control Cells Kit.  PN: 2406   50 tests*

CD4 Sure Control Cells

CD45+ cell populations in CD4sure control cells

CD4 Sure Control Cells are a preparation of lyophilized peripheral blood mononuclear cells that have been isolated from normal human peripheral blood using density gradient separation.

The preparation contains  a known number of CD4-T and CD45-positive mononuclear cells, at the time of shipping expressing surface antigens representative of those found in normal whole blood.

This kit is a research material product that has not entered manufacturing processes. All stability and data values for this item have been determined from research studies. Not for in vivo or in vitro diagnostic use.

Assays

ImmunoSite Technologies (IST) specializes in targeted pharma services, developing, standardizing and performing validated GLP assays for immune monitoring and surveillance; assessing immune status and function associated with cell-free and cell mediated immunity.

• “Off The Shelf” Assays and Cocktails

  • Five-color optimized and automated Intracellular Cytokine Cocktails: Th1, Th2 and Th17
  • Newly automated cell-based assays on the Biomek liquid handler platform:
    • Immunogenicity testing
    • Cytotoxicity
    • Apoptosis
    • Proliferation
    • Leukemia & Lymphoma
    • Cell Signaling

Cell Mediated Immunity Assays

• Functional:
  • ex-vivo and in-vitro whole blood and peripheral blood mononuclear cells (automated and manual)
• Phenotyping:
  • whole blood, peripheral blood mononuclear cells, tissue exudates, spleen, pancreas (automated and manual)
• Polyspectral flow cytometry:
  • 4-15 color
• High Speed Flow Cytometry up to 100K events/second:
  • Cell Sorting
  • Analysis
• Surface Immunophenotyping
  • T, B, Natural Killer, Granulocytes, Dendritic cells, Natural Killer T cells, Gamma-Delta T cells, Monocytes, Macrophages
  • Further T cell breakdown for phenotyping
  • Naïve, Memory, Effector, Activated, Inflammatory, Regulatory T cells.
  • Antigen specific phenotyping with Class I and II Tetramers
• Intracellular Phenotypic Staining
  • TLRs, Myeloperoxidase, CD79a, alpha Defensins
  • Regulatory T cells (FoxP3)
• Intracellular Cytokines
  • TH1: IL2/IFNg/TNFa
  • TH2: IL-4/IL10
  • TH17: IL17/IL-21
  • DC1/DC2:IFNa/IL-12
• Cytotoxicity
  • Perforin, granzymes, CD107a/b
• Proliferation
  • CFSE, Ki67, BrdU, CyclinA2
• Cell Signalling Assays
  • Target phosphorylation assays, kinase, cleaved caspase assays, ZAP70.
• Functional T Regulatory Cell Assays
  • T reg cell sorting and CFSE proliferation
• MLR (Mixed Lymphocyte Reaction) for Flow
  • CFSE and cytokines
• ELISPOT
  • IFNg/IL-10

Phenotypic Assays: Standardized and Automated

• Extended WBC Differential
  • Enumeration of WBC population
• T-Cell Activation
  • CD45/CD4/CD8/CD3/CD69
  • CD45/CD38/HLA-DR/CD25/CD71
• T-Cell Differentiation
  • CD8/CD4/CD45RA/CD27/CD28
  • CD8/CD4/CD7/CD27/CD28
  • CD8/CD4/CD45RA/CD57/CD28
  • CD8/CD45RA/CD27/CD28/CD244
• Innate Cell Phenotyping
  • NK Cells & NKT Cells
  • Myeloid Dendritic Cells (MDC)
  • Plasmacytoid Dendritic Cells (PDC)
  • Gamma Delta T Cells

Functional Assays: Custom Designed, Semi-Automated

• Innate Cell Function
  • Dendritic Cell Function with IFNa and IL-12
• T Cell Function (Example Panels)
  • T Helper/Cytotoxic 1: CD3/CD4/CD8/INFy/Granzyme-B/CD107a/b
  • T Helper/Cytotoxic 2: CD3/CD4/CD8/IL-4/ Granzyme-B/CD107a/
• Cell-Free Assays
  • EIAs (for analyte or antibody)
  • Multiplexed Immunoassays (Bender bead, A²)
  • Neutralizing antibody assays
  • P24, PBMC syncitia, Receptor/co-receptor cell line-based neutralizing antibody assays for HIV

Assay Standardization and Automation

• Cell Mediated Immunity
• Functional Cellular Assays
• Multiplexed ImmunoAssays
• ELISA
• Cell Signaling Assays
• Ab Neutralization Assays

Validated in vitro Functional Assays

• Whole blood stimulation with PMA/Ionomycin
• Whole blood stimulation with CD3/CD28
• PBMC stimulation with PMA/Ionomycin
• PBMC stimulation with CD3/CD28

Validated Cellular Prep Methods

• • Manual ICS
  • Auto ICS
  • Manual phenotyping
  • Auto phenotyping
  • Manual Ficoll-Hypaque
  • Automated Ficoll-Hypaque
  • Cryopreservation and Thawing

Reagents

Reagent and Assay Design and Optimization

• • Flow Cytometry
  • Automated assay reagents
  • Control Cell Products

Validated Cellular Reagents

• • Th/c1
  • Th/c2
  • Th/c17
  • Control Cells with high temp stability*

Validated System Reagents

• • autoCLEAN*
  • autoRINSE*
  • autoFLO*
  • PBS*

Additional Services

• Manual and automated PBMC Isolations from human, non human primates, Beagles and Rats

• Custom Assay Design

• Custom Assay qualification, standardization and automation

• Particle Characterization Services

* product release 2012